Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries

Source : https://www.nature.com/articles/s41587-020-00778-3

Current methods used for measuring amino acid side-chain reactivity lack the throughput needed to screen large chemical libraries for interactions across the proteome. Here we redesigned the workflow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based probe, sample multiplexing, reduced protein starting amounts and software to boost data acquisition in real time on the mass spectrometer.