Skip to main content
  • Saved

made a Post

Efficient and Robust NK-Cell Transduction With Baboon Envelope Pseudotyped Lentivector

Efficient and Robust NK-Cell Transduction With Baboon Envelope Pseudotyped Lentivector

Source : https://doi.org/10.3389/fimmu.2019.02873

NK-cell resistance to transduction is a major technical hurdle for developing NK-cell immunotherapy. By using Baboon envelope pseudotyped lentiviral vectors (BaEV-LVs) encoding eGFP, we obtained a transduction rate of 23.0 ± 6.6% (mean ± SD) in freshly-isolated human NK-cells (FI-NK) and 83.4 ± 10.1% (mean ± SD) in NK-cells obtained from the NK-cell Activation and Expansion System (NKAES), with a sustained transgene expression for at least 21 days.

Sign In or Register Today to Access This Content